- MACVECTOR TRIM CHROMATOGRAM FOR MAC
- MACVECTOR TRIM CHROMATOGRAM SOFTWARE
- MACVECTOR TRIM CHROMATOGRAM FREE
We supply the following standard primers (most at 4 uM): Samples MUST be organized in the 8-well dimension in plates, with samples running from 01A, 01B, 01C, etc. Organize reactions to avoid gaps where possible. Please give samples and primers reasonably short names, avoiding more than a combined length of 23 characters. For shipped samples it is especially important that the sealing method be secure against leakage, and samples packed to avoid crushing. Sealing with most tapes (clear & metallic) WILL result in leakage unless the samples are dried down. Samples should be securely sealed with strip caps. Volumes for templates and primers should reflect that we use 1.4 and 1.0 ul respectively for each reaction. Reactions should be organized in 96-well PCR-type plates, or in 8-tube strip tubes such as these:ĭO NOT use flat-bottomed plates as they are difficult to pipette from.
MACVECTOR TRIM CHROMATOGRAM FREE
High throughput reactions receive a lower price because reactions are prepared using multichannel pipettors, the resulting chromatograms are not edited, and free redo reactions are limited to facility errors, not user errors. Direction for using the portal may be found here: End User Instructions.Įxternal Users:Samples may be dropped off as above, or shipped to DNA Sequencing Facility, University of Chicago, KCBD rm 1230H, 900 E 57th St, Chicago, IL, 60637īilling questions may be directed to High-Throughput Sequencing: We require a minimum of 12, but preferably 24, reactions to be provided for this service. 57th) hallway freezer outside room 1230H (north end of east corridor under the hanging shark), or at the Cummings Life Science Center 6th floor hallway (room 604, just right of the elevators) as soon as possible after submitting your request on the DNA Order Portal. Internal Users: Please drop off samples at the KCBD (900 E. Our turnaround time is 1 business day from receipt of samples (multi-plate orders may be slightly longer).įor high-throughput sequencing (24 reactions minimum) or genotyping, custom services, such as complete sequencing projects, primer design and synthesis, etc, please call us at 77. We are committed to giving every user satisfactory sequence. Users are guaranteed satisfactory sequence from their orders even if it requires resequencing new template DNA (there is a 24 reaction limit), or the failed reactions are free.Ĭlick here for our redo policy.
MACVECTOR TRIM CHROMATOGRAM SOFTWARE
For chromatogram editing, software is available for Macintosh and Windows platforms (see below). We also run user’s reactions, and genotyping (fragment analysis) samples. Furthermore, collaboration with colleagues will be easier than ever because MacVector lets you customize graphic results and export them for high-quality printing.Users provide DNA (plasmid, phage or PCR product) at a standardized concentration and custom primers if necessary, and the Facility performs cycle sequencing reactions using fluorescent dye terminators, runs the capillaries, acquires the data, and provides the sequence as chromatogram and text files. You'll be amazed at how quickly you can flow from task to task with just a few mouse clicks. With MacVector you can view the results of any analysis in graphical or text format.Īll analysis, editing, and display functions are contained within a single application, so not only will you find MacVector remarkably user-friendly but you'll also save time whether you're exploring gene relationships, predicting coding sequences, or conducting cloning experiments. MacVector provides sequence editing, primer design, Internet database searches, protein analysis, sequence confirmation, contig assembly capabilities, multiple sequence alignment, phylogenetic reconstruction, coding region analysis, and a wide variety of other functions.
MACVECTOR TRIM CHROMATOGRAM FOR MAC
MacVector is the leading package of kind for Mac and an ideal solution for researchers who want a simple but comprehensive desktop sequence analysis application.
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